Auramine O나 Auramine-rhodamine을 이용한 염색법으로 20x 또는 40x 대물렌즈로 관찰 한다 (접안렌즈 기준 200배 또는 400배). 이러한 형광염색은 Ziehl-Neelsen (ZN) 염색에 비해 민감도가 10% 높아서 관찰량이 많은 사용자에게 권고하는 것이 좋다.
ET – DAPI/FITC/TRITC/Cy5 Quad with Single Band Exciters
A version of the 89000 Quad set with a quad band emisison filter and four individual excitation filters for use in an external filter housing such as a filter wheel, similar to Chroma’s original Pinkel filter set. Allows for selective excitation of individual fluors in sample containing multiple fluors. Appropriate for live viewing or image acquisition with monochrome or color cameras.
89400 Spectrum
Chroma_89400
Filters
Type
AT350/50x
EX
ET402/15x
EX
ET490/20x
EX
ET555/25x
EX
ET645/30x
EX
89100bs
BS
89101m
EM
89400 Spec
Typical Application(s) : Widefield Microscopy
Coating : Sputter/Hard Coated
Round excitation and emission filters mounted in anodized aluminum rings 2.3mm thick, up to 25mm in diameter (for standard sizes)
Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes (for standard sizes)
*** For filters larger than 25mm and dichroics larger than 26x38mm, please contact us for pricing.
NOTE: These require the use of filter wheels or sliders, or image-splitting devices to hold multiple individual filters.