카테고리 보관물: Fluorescence

Chroma, Fluorescence, Fluorochrome

AlexaFluor 647

Spectrum Chart

AlexaFluor 647 Spectrum
Excitation (㎚) Emission (㎚)
649 666

Recommended Filter Set

  • 일반 형광 Lighting source 사용시

Filter Model No. Excitation filter (㎚) Dichroic mirror (㎚) Emission filter (㎚) Remarks
JNO-R2(B) 620/50
(595~645)		 655 (LP) 690/50
(665~715)		 Wide Excitation band,
Bandpass Emission
49006 620/60
(590~650)		 660 (LP) 700/75
(662.5~737.5)		 Very Wide Excitation band,
Bandpass Emission
49009 640/30
(625~655)		 660 (LP) 690/50
(665~715)		 Medium Excitation band,
Bandpass Emission

 

  • 레이저 Lighting source 사용시

Filter Model No. Excitation filter (㎚) Dichroic mirror (㎚) Emission filter (㎚) Remarks
49913 635/20
(625~645)		 640 (LP) 655(LP) For use with laser between 627~643㎚

Narrow Excitation band,
Longpass Emission
49914 642/20
(632~652)		 647 (LP) 705/72
(669~741)		 For use with laser between 633~650㎚

Narrow Excitation band,
Bandpass Emission

 

 

Chroma, Filter, Fluorescence

49015

Chroma Filter 49015

49015 Spectrum

FiltersType
ET605/50xEX
 T635lpxrBS
ET670/50mEM

– Filter set for Alexa Fluor 633

Excitation (㎚)Emission (㎚)
632647

Spectrum Comparison Fluor and 49015

Best with these fluorochromes

49015 Spec

  • Typical Application(s) : Widefield Microscopy
  • Coating : Sputter/Hard Coated
  • Round excitation and emission filters mounted in anodized aluminum rings 2.3mm thick, up to 25mm in diameter
  • Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes
Chroma, Fluorescence, Fluorochrome

AlexaFluor 633

Spectrum Chart

AlexaFluor 633 Spectrum

Excitation (㎚) Emission (㎚)
632 647

 

Recommended Filter Set

  • 일반 형광 Lighting source

Filter Model No. Excitation filter (㎚) Dichroic mirror (㎚) Emission filter (㎚) Remarks
49015 605/50
(580~630)		 635 (LP) 670/50
(645~695)		 Wide Excitation band,
Bandpass Emission

  • 레이저 Lighting source 

Filter Model No. Excitation filter (㎚) Dichroic mirror (㎚) Emission filter (㎚) Remarks
49913 635/20
(625~645)		 640 (LP) 655 (LP) For use with laser between 627~643㎚

Narrow Excitation band,
longpass Emission

 

 

Chroma, Fluorescence, Fluorochrome

AlexaFluor 594

Spectrum Chart

AlexaFluor 594 Spectrum

Excitation (㎚) Emission (㎚)
590 617

 

Recommended Filter Set

  • 일반 형광 Lighting source 사용시

Filter Model No. Excitation filter (㎚) Dichroic mirror (㎚) Emission filter (㎚) Remarks
JNO-R1(B) 560/40
(540~580)		 600 (LP) 635/60
(605~665)		 Wide Excitation band,
Bandpass Emission
49008 560/40
(540~580)		 600 (LP) 630/75
(592.5~667.5)		 Wide Excitation band,
Bandpass Emission
49306 580/25
(567.5~592.5)		 600 (LP) 625/30
(610~640)		 Narrow Excitation band,
Bandpass Emission

 

  • 레이저 Lighting source 사용시

Filter Model No. Excitation filter (㎚) Dichroic mirror (㎚) Emission filter (㎚) Remarks
49911 594/10
(589~599)		 594 (LP) 645/75
(607.5~682.5)		 For use with laser 594㎚

Narrow Excitation band,
Bandpass Emission

 

 

Chroma, Fluorescence, Fluorochrome

AlexaFluor 568

Spectrum Chart

AlexaFluor 568 Spectrum

Excitation (㎚) Emission (㎚)
578 603

 

Recommended Filter Set

  • 일반 형광 Lighting source

Filter Model No. Excitation filter (㎚) Dichroic mirror (㎚) Emission filter (㎚) Remarks
JNO-R1(B) 560/40
(540~580)		 600 (LP) 635/60
(605~665)		 Wide Excitation band,
Bandpass Emission
49008 560/40
(540~580)		 600 (LP) 630/75
(592.5~667.5)		 Wide Excitation band,
Bandpass Emission
49031 569/25
(556.5~581.5)		 588 (LP) 615/40
(595~635)		 Narrow Excitation band,
Bandpass Emission

 

  • 레이저 Lighting source 

Filter Model No. Excitation filter (㎚) Dichroic mirror (㎚) Emission filter (㎚) Remarks
49909 561/10
(556~566)		 532 (LP) 600/50
(575~625)		 For use with laser between 529~535㎚

Narrow Excitation band,
Bandpass Emission
49910 561/10
(556~566)		 514 (LP) 630/70
(595~665)		 For use with laser between 557~563㎚

Narrow Excitation band,
Bandpass Emission

 

 

Chroma, Filter, FISH, Fluorescence

49031

Chroma Filter 49031

49301 Spectrum

FiltersType
ET569/25xEX
 T588lpxrBS
ET615/40mEM

– Filter set for Alexa Fluor 568, Cy3.5

Excitation (㎚)Emission (㎚)
578603

This is a narrow-band filter set intended for applications where multiple fluorochromes are present in a sample and spectral separation from neighboring fluorochromes is essential. The 49008 set will provide a brighter signal, but may provide less effective spectral separation.

Spectrum Comparison Fluor and 49031

AlexaFluor 568 VS 49031
AlexaFluor 568 VS 49031

Best with these fluorochromes

49031 Spec

  • Typical Application(s) : Widefield Microscopy
  • Coating : Sputter/Hard Coated
  • Round excitation and emission filters mounted in anodized aluminum rings 2.3mm thick, up to 25mm in diameter
  • Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes
Chroma, Fluorescence, Fluorochrome

AlexaFluor 555

Spectrum Chart

AlexaFluor 555 Spectrum

Excitation (㎚) Emission (㎚)
553 568

 

Recommended Filter Set

  • 일반 형광 Lighting source 사용시

Filter Model No. Excitation filter (㎚) Dichroic mirror (㎚) Emission filter (㎚) Remarks
JNO-G(B) 540/25
(527.5~552.5)		 565 (LP) 605/55
(577.5~632.5)		 Narrow Excitation band,
Bandpass Emission
49004 545/25
(532.5~557.5)		 565 (LP) 605/70
570~640)		 Narrow Excitation band,
Bandpass Emission
49305 546/22
(535~557)		 565 (LP) 590/33
(573.5~606.5)		 Narrow Excitation band,
Bandpass Emission

 

  • 레이저 Lighting source 사용시

Filter Model No. Excitation filter (㎚) Dichroic mirror (㎚) Emission filter (㎚) Remarks
49907 532/10
(527~537)		 532 (LP) 575/50
(550~600)		 For use with laser between 529~535㎚

Narrow Excitation band,
Bandpass Emission
49908 532/10
(527~537)		 532 (LP) 590/50
(565~615)		 For use with laser between 529~535㎚

Narrow Excitation band,
Bandpass Emission

 

 

 

 

 

 

 

 

 

Chroma, Fluorescence, Fluorochrome

AlexaFluor 546

Spectrum Chart

AlexaFluor 546 Spectrum

Excitation (㎚) Emission (㎚)
556 573

Recommended Filter

  • 일반 형광 Lighting source 사용시

Filter Model No. Excitation filter (㎚) Dichroic mirror (㎚) Emission filter (㎚) Remarks
JNO-G(B) 540/25
(527.5~552.5)		 565 (LP) 605/55
(577.5~632.5)		 Narrow Excitation band,
Bandpass Emission
49004 545/25
(532.5~557.5)		 565 (LP) 605/70
570~640)		 Narrow Excitation band,
Bandpass Emission
49305 546/22
(535~557)		 565 (LP) 590/33
(573.5~606.5)		 Narrow Excitation band,
Bandpass Emission

 

  • 레이저 Lighting source 사용시

Filter Model No. Excitation filter (㎚) Dichroic mirror (㎚) Emission filter (㎚) Remarks
49908 532/10
(527~537)		 532 (LP) 590/50
(565~615)		 For use with laser between 529~535㎚

Narrow Excitation band,
Longpass Emission
49909 561/10
(556~566)		 561 (LP) 600/50
(575~625)		 For use with laser between 557~563㎚

Narrow Excitation band,
Bandpass Emission

 

 

Chroma, Filter, Fluorescence

49907

Chroma Filter 49907

49907 Spectrum

Chroma_49007
Chroma_49007
FiltersType
ZET532/10xEX
 ZT532rdcBS
ET575/50mEM

– Filter set of 532nm Laser Bandpass Set for mKO, mOrange2

For use with lasers between 529-535㎚

Excitation (㎚)Emission (㎚)
531554

Spectrum Comparison Fluor and 49907

AlexaFluor 532 VS 49907
AlexaFluor 532 VS 49907

49907 Spec

  • Typical Application(s) : Laser (Non-TIRF)
  • Coating : Sputter/Hard Coated
  • Round excitation and emission filters mounted in anodized aluminum rings 2.3mm thick, up to 25mm in diameter
  • Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes