카테고리 보관물: Fluorescence

Chroma, Fluorescence, Fluorochrome

mCherry

Spectrum Chart

mCherry Spectrum
mCherry Spectrum
Excitation (㎚)Emission (㎚)
587610

Recommended Filter set

  • 일반 형광 Lighting source 사용시
Filter Model No.Excitation filter (㎚)Dichroic mirror (㎚)Emission filter (㎚)Remarks
JNO-R1(B)560/40
(540~580)		600 (LP)635/60
(605~665)		Wide Excitation band,
Bandpass Emission
49008560/40
(540~580)		600 (LP)630/75
(592.5~667.5)		Wide Excitation band,
Bandpass Emission
49306580/25
(567.5~592.5)		600 (LP)625/30
(610~640)		Narrow Excitation band,
Bandpass Emission
  • 레이저 Lighting source 사용시
Filter Model No.Excitation filter (㎚)Dichroic mirror (㎚)Emission filter (㎚)Remarks
49910561/10
(556~565)		561 (LP)630/70
(595~665)		For use with laser between 557~563㎚ Narrow Excitation band,
Bandpass Emission
49911594/10
(589~599)		594 (LP)645/75
(607.5~682.5)		For use with laser between 591~596㎚ Narrow Excitation band,
Bandpass Emission

Information

mCherry is a fluorophore (a fluorescent protein) used as a marker when tagged to molecules and cell components. mCherry and the majority of red fluorescent proteins derive from the protein drFP583 (commonly known as DsRed) isolated from Discosoma sea anemones, while other fluorescent proteins in the green range are often variants of jellyfish GFP. DsRed is a tetramer optimally excited at 558 ㎚ and has an emission maximum at 583 ㎚.

mCherry는 형광 물질 (형광 단백질)로서 분자 및 세포 구성 요소에 태그를 부착 할 때 marker 로 사용됩니다. mCherry와 대다수의 적색 형광 단백질은 Discosoma 말미잘에서 분리된 단백질 drFP583 (일반적으로 DsRed라고 함)에서 유래한 반면, 녹색 범위의 다른 형광 단백질은 종종 해파리 GFP의 변종입니다. DsRed는 558 ㎚에서 최적으로 여기되고, 583 ㎚ 에서 최대 방출을 갖는 4 량체입니다. 

Chroma, Fluorescence, Fluorochrome

EBFP

Spectrum Chart

BFP Spectrum

Excitation (㎚) Emission (㎚)
385 448
Recommended Filter set

  • 일반 형광 Lighting source 사용시

Filter Model No. Excitation filter (㎚) Dichroic mirror (㎚) Emission filter (㎚) Remarks
49021 405/20
(395~415)		 425 (LP) 460/50
(435~485)		 Narrow Excitation band,
Bandpass Emission
49028 395/25
(382.5~407.5)		 425 (LP) 460/50
(435~485)		 Narrow Excitation band,
Bandpass Emission

 

  • 레이저 Lighting source 사용시

Filter Model No. Excitation filter (㎚) Dichroic mirror (㎚) Emission filter (㎚) Remarks
49901 405/20
(395~415)		 405 (LP) 460/50
(435~485)		 For use with laser between 400~410㎚

Narrow Excitation band,
Bandpass Emission

Information

Fluorescent proteins, like EBFP, can be used as protein “tags” to study the subcellular localization of proteins and/or their translocation upon stimulation or as markers for transfection in transient and stable expression systems.

EBFP와 같은 형광 단백질은 자극이나 전이를 위한 안정적인 marker 로서 또는 그들의 일시적인 발현의 하위 세포를 연구하기 위해 단백질 “태그” 로 사용될 수 있습니다.

 

Chroma, Filter, Fluorescence, JNOPTIC

JNO-G(L)

JNOPTIC Filter JNO-G(L)

Download Catalog _ JNO-G(L)

– Filter set for TRITC / Cy3 Longpass
– Chroma Filter Model No. : 19004

JNO-G(L) Spectrum

JNO-G(L)
Standard Green Excitaion filter (Long Pass type)
FiltersType
AT540/25xEX
AT565DCBS
AT575lpEM

주요 형광 색소(Fluorochromes)와 스펙트럼 비교

* TRITC VS JNO-G(L)

JNO-G(L)필터를 이용한 Sample Image 촬영

JNO-G(L)를 사용하여 보는 TRITC 형광 이미지
Filter Set : JNO-G(L)
Camera : AcquCAM 23GR2
C-mount adapter : 1x
Lens : UPLAPO40X
Sample : bovine pulmonary artery endothelial cell, BPAEC
JNO-G(L)를 사용하여 보는 TRITC 형광 이미지
Filter Set : JNO-G(L)
Camera : AcquCAM 5G
C-mount adapter : 1x
Lens : UPLAPO40X
Sample : bovine pulmonary artery endothelial cell, BPAEC
JNO-G(L)를 사용하여 보는 TRITC 형광 이미지
Filter Set : JNO-G(L) Camera : AcquCAM 3G C-mount adapter : 1x Lens : UPLAPO40X Sample : bovine pulmonary artery endothelial cell, BPAEC

JNO-G(L)필터에 적합한 형광색소

  • TRITC
  • Cy3
  • R-phycoerythrin

JNO-G(L) Spec

  • Typical Application(s) : Widefield Microscopy
  • Coating : Sputter/Hard Coated
  • Round excitation and emission filters mounted in anodized aluminum rings 2.3mm thick, up to 25mm in diameter (for standard sizes)
  • Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes (for standard sizes)

*** For filters larger than 25mm and dichroics larger than 26x38mm, please contact us for pricing.

Chroma, Filter, Fluorescence, JNOPTIC

JNO-B(L)

JNOPTIC Filter JNO-B(L)

Download Catalog _ JNO-B(L)

– Filter set for GFP / FITC Longpass
– Chroma Filter Model No. : 19002

JNO-B(L) Spectrum

Blue Excitation, Long Pass Emission
JNO-B(L) Spectrum
FiltersType
AT480/30xEX
 AT505DCBS
AT515lpEM

주요 형광 색소(Fluorochromes)와 스펙트럼 비교

 * FITC VS JNO-B(L)

FITC VS JNO-B(L)

 * GFP VS JNO-B(L)

GFP VS JNO-B(L)
GFP VS JNO-B(L)

JNO-B(L)필터를 이용한 Sample Image 촬영

Sample : bovine pulmonary artery endothelial cell, BPAEC Filter Set : JNO-B(L) Camera : AcquCAM 23GR2 C-mount adapter : 1X Lens : UPLNAPO40X
AcquCAM 3G BX51 UPlanApo40x JNO-B(L) 1X
Sample : bovine pulmonary artery endothelial cell, BPAEC Filter Set : JNO-B(L) Camera : AcquCAM 3G C-mount adapter : 1X Lens : UPLNAPO40X
Fluorescence Image by AcquCAM 5G
Sample : bovine pulmonary artery endothelial cell, BPAEC Filter Set : JNO-B(L) Camera : AcquCAM 5G C-mount adapter : 1X Lens : UPLNAPO40X

JNO-B(L)필터에 적합한 형광색소

  • Acridine Orange + DNA and RNA
  • Auramine-rhodamine
  • JC-1 non-ratiometric

JNO-B(L) Spec

  • Typical Application(s) : Widefield Microscopy
  • Coating : Sputter/Hard Coated
  • Round excitation and emission filters mounted in anodized aluminum rings 2.3mm thick, up to 25mm in diameter (for standard sizes)
  • Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes (for standard sizes)

*** For filters larger than 25mm and dichroics larger than 26x38mm, please contact us for pricing.

Chroma, Filter, Fluorescence, JNOPTIC

JNO-U(L)

Download Catalog _ JNO-U(L)

– Filter set for UV/DAPI Longpass
– Chroma Filter Model No. : 19000

JNO-U(L) Spectrum

JNO-U(L) Spectrum
FiltersType
AT375/28xEX
AT415DCBS
AT435lpEM

주요 형광 색소(Fluorochromes)와 스펙트럼 비교

* DAPI VS JNO-U(L)

DAPI VS JNO-U(L)

JNO-U(L)필터를 이용한 Sample Image 촬영

DAPI
Sample : bovine pulmonary artery endothelial cell, BPAEC
Lens : UPLNAPO40X, C-mount adapter : 1X
Camera : AcquCAM 23GR2, Filter Set : JNO-U(L)
DAPI
Sample : bovine pulmonary artery endothelial cell, BPAEC
Lens : UPLNAPO40X, C-mount adapter : 1X
Camera : AcquCAM 23S2, Filter Set : JNO-U(L)
DAPI
Sample : bovine pulmonary artery endothelial cell, BPAEC
Lens : UPLNAPO40X, C-mount adapter : 1X
Camera : AcquCAM 3G, Filter Set : JNO-U(L)
DAPI
Sample : bovine pulmonary artery endothelial cell, BPAEC
Lens : UPLNAPO40X, C-mount adapter : 1X
Camera : AcquCAM 5G, Filter Set : JNO-U(L)

JNO-U(L)에 적합한 형광색소

  • Calcofluor White
  • DAPI

JNO-U(L) Spec

  • Typical Application(s) : Widefield Microscopy
  • Coating : Sputter/Hard Coated
  • Round excitation and emission filters mounted in anodized aluminum rings 2.3mm thick, up to 25mm in diameter (for standard sizes)
  • Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes (for standard sizes)

*** For filters larger than 25mm and dichroics larger than 26x38mm, please contact us for pricing.

Chroma, Filter, Fluorescence

JNO-R2(B)

JNOPTIC Filter  JNO-R2(B)

Download Catalog_JNO-R2(B)

– Filter set for Cy5 / AlexaFluor 647 / Draq5
– Chroma Filter Model No. : 39007

JNO-R2(B) Spectrum

JNO-R2(B)
JNO-R2(B)
FiltersType
 AT620/50xEX
AT655DCBS
AT690/50mEM

주요 형광 색소(Fluorochromes)와 스펙트럼 비교

Cy5 VS JNO-R2(B)
Cy5 VS JNO-R2(B)
JNO-R2(B) VS AF647
JNO-R2(B) VS AF647
JNO-R2(B) VS Draq5
JNO-R2(B) VS Draq5

JNO-R2(B)필터에 적합한 형광색소

JNO-R2(B) Spec

  • Typical Application(s) : Widefield Microscopy
  • Coating : Sputter/Hard Coated
  • Round excitation and emission filters mounted in anodized aluminum rings 2.3mm thick, up to 25mm in diameter (for standard sizes)
  • Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes (for standard sizes)

*** For filters larger than 25mm and dichroics larger than 26x38mm, please contact us for pricing.

Chroma, Filter, Fluorescence, JNOPTIC

JNO-R1(B)

JNOPTIC Filter JNO-R1(B)

Download Catalog_JNO-R1(B)

– Filter set for Texas Red / mCherry / AlexaFluor 594
– Chroma Filter Model No. : 39010

JNO-R1(B) Spectrum

JNO-R1(B)
JNO-R1(B)
FiltersType
AT560/40xEX
AT600DCBS
AT635/60mEM

주요 형광 색소(Fluorochromes)와 스펙트럼 비교

Texas Red VS JNO-R1(B)
Texas Red VS JNO-R1(B)
mCherry VS JNO-R1(B)
mCherry VS JNO-R1(B)
Alexa Fluor 594 VS JNO-R1(B)
Alexa Fluor 594 VS JNO-R1(B)

JNO-R1(B)필터에 적합한 형광색소

JNO-R1(B) Spec

  • Typical Application(s) : Widefield Microscopy
  • Coating : Sputter/Hard Coated
  • Round excitation and emission filters mounted in anodized aluminum rings 2.3mm thick, up to 25mm in diameter (for standard sizes)
  • Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes (for standard sizes)

*** For filters larger than 25mm and dichroics larger than 26x38mm, please contact us for pricing.

Chroma, Filter, Fluorescence, JNOPTIC

JNO-G(B)

JNOPTIC Filter JNO-G(B)

Download Catalog _ JNO-G(B)

– Filter set for TRITC / Cy3 / TagRFP / AlexaFluor546
– Chroma Filter Model No. : 39004

JNO-G(B) Spectrum

JNO-G(B)
Standard Green Excitaion filter (Bans Pass type)
FiltersType
AT540/25xEX
AT565DCBS
AT605/55mEM

주요 형광 색소(Fluorochromes)와 스펙트럼 비교

* TRITC VS JNO-G(B)

TRITC VS JNO-G(B) Spetctrum Data
TRITC에 사용했을때 JNO-G(B)

 * Cy3 VS JNO-G(B)

 * AlexaFluor546 VS JNO-G(B)

JNO-G(B) VS AF594
AF594에 사용되는 JNO-G(B)의 스펙트럼 비교

JNO-G(B)필터를 이용한 Sample Image 촬영

JNO-G(B)를 사용하여 보는 TRITC 형광 이미지
Filter Set : JNO-G(B)
Camera : AcquCAM 3G
C-mount adapter : 1x
Lens : UPLAPO20X
Sample : bovine pulmonary artery endothelial cell, BPAEC
대체 텍스트
Filter Set : JNO-G(B)
Camera : AcquCAM 5G
C-mount adapter : 1x
Lens : UPLAPO40X
Sample : bovine pulmonary artery endothelial cell, BPAEC
JNO-G(B)를 사용하여 보는 TRITC 형광 이미지
Filter Set : JNO-G(B)
Camera : AcquCAM 23GR2
C-mount adapter : 1x
Lens : UPLAPO20X
Sample : bovine pulmonary artery endothelial cell, BPAEC

JNO-G(B)필터에 적합한 형광색소

JNO-G(B) Spec

  • Typical Application(s) : Widefield Microscopy
  • Coating : Sputter/Hard Coated
  • Round excitation and emission filters mounted in anodized aluminum rings 2.3mm thick, up to 25mm in diameter (for standard sizes)
  • Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes (for standard sizes)

*** For filters larger than 25mm and dichroics larger than 26x38mm, please contact us for pricing.

Chroma, Filter, Fluorescence, JNOPTIC

JNO-B(B)

JNOPTIC Filter JNO-B(B)

Download Catalog _ JNO-B(B)

– Filter set for EGFP / FITC / Cy2 / AlexaFluor488
– Chroma Filter Model No. : 39002

JNO-B(B) Spectrum

FiltersType
AT480/30xEX
 AT505DCBS
AT535/40mEM

주요 형광 색소(Fluorochromes)와 스펙트럼 비교

* FITC VS JNO-B(B)

FITC VS JNO-B(B)

 * GFP VS JNO-B(B)

JNO-B(B) VS GFP spectrum 비교
JNO-B(B) VS GFP spectrum chart

 * AlexaFluor488 VS JNO-B(B)

JNO-B(B) VS AlexaFluor 488
JNO-B(B) VS AlexaFluor 488

JNO-B(B)필터를 이용한 Sample Image 촬영

Fluorescence Image by AcquCAM 23GR2
Sample : bovine pulmonary artery endothelial cell, BPAEC Filter Set : JNO-B(B) Camera : AcquCAM 23GR2 C-mount adapter : 1X Lens : PlanApo60xWLSM
Fluorescence Image by AcquCAM 3G
Sample : bovine pulmonary artery endothelial cell, BPAEC Filter Set : JNO-B(B) Camera : AcquCAM 3G C-mount adapter : 1X Lens : PlanApo60xWLSM
Fluorescence Image by AcquCAM 5G
Sample : bovine pulmonary artery endothelial cell, BPAEC Filter Set : JNO-B(B) Camera : AcquCAM 5G C-mount adapter : 1X Lens : UPLNAPO40X

JNO-B(B)필터에 적합한 형광색소

JNO-B(B) Spec

  • Typical Application(s) : Widefield Microscopy
  • Coating : Sputter/Hard Coated
  • Round excitation and emission filters mounted in anodized aluminum rings 2.3mm thick, up to 25mm in diameter (for standard sizes)
  • Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes (for standard sizes)

*** For filters larger than 25mm and dichroics larger than 26x38mm, please contact us for pricing. ( Tel. 02-3473-4188)

Chroma, Filter, Fluorescence, JNOPTIC

JNO-U(B)

Download Catalog _ JNO-U(B)

– Filter set for DAPI / Hoechst / AlexaFluor350
– Chroma Filter Model No. : 39000

JNO-U(B) Spectrum

JNO-U(B)
Filters Type
 AT375/28x EX
AT415DC BS
 AT460/50m EM

주요 형광 색소(Fluorochromes)와 스펙트럼 비교

* DAPI VS JNO-U(B)

DAPI VS JNO-U(B)

* Hoechst VS JNO-U(B)

JNO-U(B) VS Hoechst

 * AlexaFluor350 VS JNO-U(B)

JNO-U(B) VS AF350

JNO-U(B)필터를 이용한 Sample Image 촬영

DAPI
Sample : bovine pulmonary artery endothelial cell, BPAEC
Camera : AcquCAM 23GR2, Filter Set : JNO-U(B)
Objective lens : UPLNAPO40X, C-mount adapter : 1X
AcquCAM 23GR2 fluorescence Image
DAPI, FITC, TRITC
Sample : bovine pulmonary artery endothelial cell, BPAEC
Camera : AcquCAM 23GR2, Filter Set : JNO-U(B),B(B),G(B)
Objective lens : UPLNAPO40X, C-mount adapter : 1X (OLYMPUS)
Above three images are merged and processed using JNO-ARM
DAPI, FITC, TRITC
Sample : bovine pulmonary artery endothelial cell, BPAEC
Camera : AcquCAM 3G, Filter Set : JNO-U(B),B(B),G(B)
Objective lens : UPLNAPO40X, C-mount adapter : 1X
Above three images are merged and processed using JNO-ARM

JNO-U(B) 필터에 적합한 형광색소

JNO-U(B) Spec

  • Typical Application(s) : Widefield Microscopy
  • Coating : Sputter/Hard Coated
  • Round excitation and emission filters mounted in anodized aluminum rings 2.3mm thick, up to 25mm in diameter (for standard sizes)
  • Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes (for standard sizes)

*** For filters larger than 25mm and dichroics larger than *** 26x38mm, please contact us for pricing.