월별 글 목록: 2018년 4월월

260607_Photonics, Chroma, Filter, Fluorescence

49003

Chroma Filter 49003

49003 Spectrum

Chroma_49003
Chroma_49003
FiltersType
ET500/20xEX
 T515LPBS
ET535/30mEM

– Filter set for EYFP

  • Main Fluorochromes : YFP
Excitation (㎚)Emission (㎚)
514527

Spectrum Comparison Fluor and 49003

EYFP VS 49003
EYFP VS 49003

Best with these fluorochromes

49003 Spec

  • Typical Application(s) : Widefield Microscopy
  • Coating : Sputter/Hard Coated
  • Round excitation and emission filters mounted in anodized aluminum rings 2.3mm or 3.5mm thick, up to 25mm in diameter
  • Rectangular dichroics up to 26x38mm or 25.5×36, 1mm thick, to fit standard microscope manufacturer filter cubes
260607_Photonics, Chroma, Fluorescence, Fluorochrome

mCherry

Spectrum Chart

mCherry Spectrum
mCherry Spectrum
Excitation (㎚)Emission (㎚)
587610

Recommended Filter set

  • 일반 형광 Lighting source 사용시
Filter Model No.Excitation filter (㎚)Dichroic mirror (㎚)Emission filter (㎚)Remarks
JNO-R1(B)560/40
(540~580)		600 (LP)635/60
(605~665)		Wide Excitation band,
Bandpass Emission
49008560/40
(540~580)		600 (LP)630/75
(592.5~667.5)		Wide Excitation band,
Bandpass Emission
49306580/25
(567.5~592.5)		600 (LP)625/30
(610~640)		Narrow Excitation band,
Bandpass Emission
  • 레이저 Lighting source 사용시
Filter Model No.Excitation filter (㎚)Dichroic mirror (㎚)Emission filter (㎚)Remarks
49910561/10
(556~565)		561 (LP)630/70
(595~665)		For use with laser between 557~563㎚ Narrow Excitation band,
Bandpass Emission
49911594/10
(589~599)		594 (LP)645/75
(607.5~682.5)		For use with laser between 591~596㎚ Narrow Excitation band,
Bandpass Emission

Information

mCherry is a fluorophore (a fluorescent protein) used as a marker when tagged to molecules and cell components. mCherry and the majority of red fluorescent proteins derive from the protein drFP583 (commonly known as DsRed) isolated from Discosoma sea anemones, while other fluorescent proteins in the green range are often variants of jellyfish GFP. DsRed is a tetramer optimally excited at 558 ㎚ and has an emission maximum at 583 ㎚.

mCherry는 형광 물질 (형광 단백질)로서 분자 및 세포 구성 요소에 태그를 부착 할 때 marker 로 사용됩니다. mCherry와 대다수의 적색 형광 단백질은 Discosoma 말미잘에서 분리된 단백질 drFP583 (일반적으로 DsRed라고 함)에서 유래한 반면, 녹색 범위의 다른 형광 단백질은 종종 해파리 GFP의 변종입니다. DsRed는 558 ㎚에서 최적으로 여기되고, 583 ㎚ 에서 최대 방출을 갖는 4 량체입니다. 

260607_Photonics, Chroma, Fluorescence, Fluorochrome

EBFP

Spectrum Chart

BFP Spectrum

Excitation (㎚) Emission (㎚)
385 448
Recommended Filter set

  • 일반 형광 Lighting source 사용시

Filter Model No. Excitation filter (㎚) Dichroic mirror (㎚) Emission filter (㎚) Remarks
49021 405/20
(395~415)		 425 (LP) 460/50
(435~485)		 Narrow Excitation band,
Bandpass Emission
49028 395/25
(382.5~407.5)		 425 (LP) 460/50
(435~485)		 Narrow Excitation band,
Bandpass Emission

 

  • 레이저 Lighting source 사용시

Filter Model No. Excitation filter (㎚) Dichroic mirror (㎚) Emission filter (㎚) Remarks
49901 405/20
(395~415)		 405 (LP) 460/50
(435~485)		 For use with laser between 400~410㎚

Narrow Excitation band,
Bandpass Emission

Information

Fluorescent proteins, like EBFP, can be used as protein “tags” to study the subcellular localization of proteins and/or their translocation upon stimulation or as markers for transfection in transient and stable expression systems.

EBFP와 같은 형광 단백질은 자극이나 전이를 위한 안정적인 marker 로서 또는 그들의 일시적인 발현의 하위 세포를 연구하기 위해 단백질 “태그” 로 사용될 수 있습니다.